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The Arthur Ross Terrace will be closed this morning, Tuesday, October 21, for a private cultural observance. You many observe smoke and/or fire coming from the Terrace at that time. The FDNY has been notified in advance, and all safety precautions are in place. The Terrace will reopen at 1 pm.

AMPure Cleanup: Robot or by hand

1. Transfer 18uL AMPure to an Eppendorf plate.

2. Add 10uL PCR product and mix.

3. Let sit 5 minutes.

4. Place on magnet, let sit 5-10 minutes.

5. Remove entire volume, leaving beads, discard liquid.

6. Add 200uL 70% Ethanol, let sit 30 seconds, flip while still on magnet to remove ethanol.

7. Repeat Ethanol wash.

8. Take plate off magnet; let air dry for 12-25 minutes.

9. Add 40uL H2O, mixing well, to re-suspend the DNA.

10. When you are ready to set up your sequencing reactions,

11. Place plate on magnet for 10 minutes.

Faint band concentration:
After AMPure cleanup, place plate on magnet for 10 minutes and transfer product to a new tube or plate. Evaporate to dryness on DNA speedvac. Re-suspend in 10uL H2O and proceed to sequencing reaction.

American Museum of Natural History

Central Park West at 79th Street
New York, NY 10024-5192
Phone: 212-769-5100

Open daily from 10 am - 5:45 pm
except on Thanksgiving and Christmas
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