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Set up in Eppendorf plate.

5uL cleaned PCR product
1uL Big Dye Extender Buffer
1uL 3.2 uM primer
1uL Big Dye
Seal with wax or heat seal.

Thermal profile:
Basic program
94 C :30
50 C 1:00
60 C 4:00
35 cycles
hold at 4 C

American Museum of Natural History

Central Park West at 79th Street
New York, NY 10024-5192
Phone: 212-769-5100

Open daily from 10 am-5:45 pm
except on Thanksgiving and Christmas
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